409-ml (r&d systems)
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r&d systems
409-ml
409 Ml, supplied by r&d systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il-9/Recombinant+Mouse+IL-9+Protein/pmc11440512-62-0-4
Average 93 stars, based on 16 article reviews
409 Ml, supplied by r&d systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il-9/Recombinant+Mouse+IL-9+Protein/pmc11440512-62-0-4
Average 93 stars, based on 16 article reviews
409-ml - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "MicroRNA-19b exacerbates systemic sclerosis through promoting Th9 cells"
Article Title: MicroRNA-19b exacerbates systemic sclerosis through promoting Th9 cells
Journal: Cell reports
doi: 10.1016/j.celrep.2024.114565
Figure Legend Snippet:
Techniques Used: Purification, Control, Virus, Plasmid Preparation, Expressing, Retroviral, Recombinant, Activation Assay, Modification, Infection, Transfection, Lysis, Extraction, Western Blot, Electron Microscopy, Cell Isolation, Staining, Mutagenesis, Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Gene Expression, cDNA Synthesis, Sequencing, Software, Real-time Polymerase Chain Reaction
Related Articles
Western Blot:Article Title: Interleukin-9 Regulates NF-kB-Mediated Activation of Astrocytes in Multiple Sclerosis Brain Article Snippet: .. Table 2 Antibodies Used for Experiments Primary antibody Origin Target Dilution Source Application GAPDH Mouse (mAB; IgG 1 κ) Glyceraldehyde-3-phosphate dehydrogenase 1:1,000 Santa Cruz Biotechnology, INC WB GFAP Mouse (mAB, IgG1) Glial fibrillary acidic protein 1:200 Cell Signaling Technology, MA, United States IHC GFAP Rabbit (pAB) Glial fibrillary acidic protein 1:500 Invitrogen, MA, United States IF IBA1 Rabbit (pAB) Ionized calcium-binding adapter molecule 1 1:400 Immunologic Sciences, Rome, Italy IF MBP Rabbit (pAB) Myelin basic protein 1:200 Cell Signaling Technology, MA, United States IF IgG Rabbit (pAB, IgG) — 1:5,000 Millipore Sigma, MA, United States ChIP IL-9R Mouse (mAB, IgG) Interleukin-9 receptor 1:100 MAB290, R&D Systems, Minneapolis, United States IHC/IF Isotype IgG Mouse (mAB, IgG) — 1:100 MAB002, R&D Systems, Minneapolis, United States IF IL-9R Rabbit (pAB) Interleukin-9 receptor 1:500 ABCAM, Cambridge, United Kingdom WB NF-kB p65 Mouse (mAB, IgG2b) Nuclear factor-kB p65/RelA 1:800 Cell Signaling Technology, MA, United States IF NF-κB p65 Rabbit (mAB, IgG) Nuclear factor-kB p65/RelA 1:1,000 Cell Signaling Technology, MA, United States ChIP NF-kB p65 Rabbit (mAB, IgG) Nuclear factor-kB p65/RelA 1:700 ABCAM, Cambridge, United Kingdom IF Phospho-IKKα/β Rabbit (mAB, IgG) Phospho- IkB kinase α/β 1:1,000 Cell Signaling Technology, MA, United States WB Phospho-NF-kB p65 Rabbit (mAB, IgG) Phospho-nuclear factor-kB p65 1:1,000 Cell Signaling Technology, MA, United States WB GM-CSF Rat (mAB, IgG2aκ) Granulocyte-macrophage colonystimulating factor 1:30 Miltenyi Biotec, Bergisch Gladbach, Germany FC β-Actin Mouse (mAB) Beta-actin 1:1,000 Abgent, CA, United States WB List of antibodies used for immunohistochemistry (IHC), immunofluorescence (IF), chromatin immune precipitation (ChIP), western blot (WB), and flow cytometry (FC). .. Immunohistochemistry:Article Title: Interleukin-9 Regulates NF-kB-Mediated Activation of Astrocytes in Multiple Sclerosis Brain Article Snippet: .. Table 2 Antibodies Used for Experiments Primary antibody Origin Target Dilution Source Application GAPDH Mouse (mAB; IgG 1 κ) Glyceraldehyde-3-phosphate dehydrogenase 1:1,000 Santa Cruz Biotechnology, INC WB GFAP Mouse (mAB, IgG1) Glial fibrillary acidic protein 1:200 Cell Signaling Technology, MA, United States IHC GFAP Rabbit (pAB) Glial fibrillary acidic protein 1:500 Invitrogen, MA, United States IF IBA1 Rabbit (pAB) Ionized calcium-binding adapter molecule 1 1:400 Immunologic Sciences, Rome, Italy IF MBP Rabbit (pAB) Myelin basic protein 1:200 Cell Signaling Technology, MA, United States IF IgG Rabbit (pAB, IgG) — 1:5,000 Millipore Sigma, MA, Chromatin Immunoprecipitation:Article Title: Interleukin-9 Regulates NF-kB-Mediated Activation of Astrocytes in Multiple Sclerosis Brain Article Snippet: .. Table 2 Antibodies Used for Experiments Primary antibody Origin Target Dilution Source Application GAPDH Mouse (mAB; IgG 1 κ) Glyceraldehyde-3-phosphate dehydrogenase 1:1,000 Santa Cruz Biotechnology, INC WB GFAP Mouse (mAB, IgG1) Glial fibrillary acidic protein 1:200 Cell Signaling Technology, MA, United States IHC GFAP Rabbit (pAB) Glial fibrillary acidic protein 1:500 Invitrogen, MA, United States IF IBA1 Rabbit (pAB) Ionized calcium-binding adapter molecule 1 1:400 Immunologic Sciences, Rome, Italy IF MBP Rabbit (pAB) Myelin basic protein 1:200 Cell Signaling Technology, MA, United States IF IgG Rabbit (pAB, IgG) — 1:5,000 Millipore Sigma, MA, Immunofluorescence:Article Title: Interleukin-9 Regulates NF-kB-Mediated Activation of Astrocytes in Multiple Sclerosis Brain Article Snippet: .. Table 2 Antibodies Used for Experiments Primary antibody Origin Target Dilution Source Application GAPDH Mouse (mAB; IgG 1 κ) Glyceraldehyde-3-phosphate dehydrogenase 1:1,000 Santa Cruz Biotechnology, INC WB GFAP Mouse (mAB, IgG1) Glial fibrillary acidic protein 1:200 Cell Signaling Technology, MA, United States IHC GFAP Rabbit (pAB) Glial fibrillary acidic protein 1:500 Invitrogen, MA, United States IF IBA1 Rabbit (pAB) Ionized calcium-binding adapter molecule 1 1:400 Immunologic Sciences, Rome, Italy IF MBP Rabbit (pAB) Myelin basic protein 1:200 Cell Signaling Technology, MA, United States IF IgG Rabbit (pAB, IgG) — 1:5,000 Millipore Sigma, MA, Flow Cytometry:Article Title: Interleukin-9 Regulates NF-kB-Mediated Activation of Astrocytes in Multiple Sclerosis Brain Article Snippet: .. Table 2 Antibodies Used for Experiments Primary antibody Origin Target Dilution Source Application GAPDH Mouse (mAB; IgG 1 κ) Glyceraldehyde-3-phosphate dehydrogenase 1:1,000 Santa Cruz Biotechnology, INC WB GFAP Mouse (mAB, IgG1) Glial fibrillary acidic protein 1:200 Cell Signaling Technology, MA, United States IHC GFAP Rabbit (pAB) Glial fibrillary acidic protein 1:500 Invitrogen, MA, United States IF IBA1 Rabbit (pAB) Ionized calcium-binding adapter molecule 1 1:400 Immunologic Sciences, Rome, Italy IF MBP Rabbit (pAB) Myelin basic protein 1:200 Cell Signaling Technology, MA, United States IF IgG Rabbit (pAB, IgG) — 1:5,000 Millipore Sigma, MA, Recombinant:Article Title: A reciprocal glial circuit calibrates injury information and governs the tissue response balance. Article Snippet: Article Article Title: Group 2 innate lymphoid cells derived IL-9 reduces macrophage apoptosis and attenuates acute lung injury in sepsis. Article Snippet: Xiaohui Zhong and Jingyi Jin have contributed equally to this work and share first authorship.. Abstract Background Group 2 innate lymphoid cells (ILC2) are the main group of tissue-resident ILCs in the lungs, which protect airway barrier integrity following infection.. Macrophages are integral to the regulation of immune homeostasis in sepsis. Enzyme-linked Immunosorbent Assay:Article Title: A reciprocal glial circuit calibrates injury information and governs the tissue response balance. Article Snippet: Article Mouse Assay:Article Title: Potent antitumor activity of a designed interleukin-21 mimic Article Snippet: .. When tumors were palpable 6 days post-tumor inoculation, mice were dosed intraperitoneally with PBS (n=5 mice), 21h10 (15 μg per mouse, n=5 mice ), Article Title: Group 2 innate lymphoid cells derived IL-9 reduces macrophage apoptosis and attenuates acute lung injury in sepsis. Article Snippet: Xiaohui Zhong and Jingyi Jin have contributed equally to this work and share first authorship.. Abstract Background Group 2 innate lymphoid cells (ILC2) are the main group of tissue-resident ILCs in the lungs, which protect airway barrier integrity following infection.. Macrophages are integral to the regulation of immune homeostasis in sepsis. Sterility:Article Title: Potent antitumor activity of a designed interleukin-21 mimic Article Snippet: .. When tumors were palpable 6 days post-tumor inoculation, mice were dosed intraperitoneally with PBS (n=5 mice), 21h10 (15 μg per mouse, n=5 mice ), other:Article Title: Mechanical force receptor Piezo1 regulates TH9 cell differentiation Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Aluminum hydroxide Sigma-Aldrich, USA Cat#239186 Antimycin A Sigma-Aldrich, USA Cat#A8674 Bovine serum albumin (BSA) Sigma-Aldrich, USA Cat#A7030 CaCl2 Merck, Germany Cat#C5670 Collagenase D Worthington, USA Cat#LS005273 CoCl2 Merck, Germany Cat#409332 Deoxyribonuclease I Beyotime Biotechnology, China Cat#D7076 DMEM Thermo Fisher Scientific, USA Cat#C11995500BT DMSO Sigma-Aldrich, USA Cat# D2650 Dooku1 MedChemExpress, USA Cat#HY-126010 Dimethyl malonate (DMM) Selleck, USA Cat#S6208 Fetal bovine serum (FBS) Every Green, Belgium Cat#11011-8611 Fibronectin Sigma-Aldrich, USA Cat#FG001 Fura-2 a.m. Sigma-Aldrich, USA Cat#F1201 Glucose Invitrogen, USA Cat#15023021 HEPES Invitrogen, USA Cat#15630130 Hoechest 33342 Beyotime Biotechnology, China Cat#C1029 KCl Thermo Fisher Scientific, USA Cat#ECCON84BT L-glutamine Sigma-Aldrich, USA Cat#G8540 Lipofectamine 3000 Thermo Fisher Scientific, USA Cat#L3000150 Methacholine Sigma-Aldrich, USA Cat#62-51-1 MgCl2 Invitrogen, USA Cat#AM9530G NaN3 Sigma-Aldrich, USA Cat#S2002 NaCl Invitrogen, USA Cat# AM9760G NaOH Merch, Germany Cat#1.09137 4% paraformaldehyde (PFA) Solarbio, China Cat#P1110 Oligomycin Sigma-Aldrich, USA Cat#495455 Ovalbumin (OVA) Sigma-Aldrich, USA Cat#S7951 Percoll GE Health, USA Cat#17-0891-01 Phorbol ester (PMA) Sigma-Aldrich, USA Cat#P8139 Protein A/G plus agarose beads Thermo Fisher Scientific, USA Cat#26159 RPMI1640 Thermo Fisher Scientific, USA Cat#C11875500BT Rotenone Sigma-Aldrich, USA Cat#R8875 |